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Biotechnology and Applied Biochemistry (2003) 38, (119–122) (Printed in Great Britain)

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A reagentless nitric oxide biosensor based on haemoglobin/polyethyleneimine film

Xinjian Liu, Libin Shang, Jiantao Pang and Genxi Li¹

Department of Biochemistry and National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, People's Republic of China

Key words: biosensor, chemically modified electrode, cyclic voltammetry, direct electrochemistry, haemoglobin, nitric oxide.

Abbreviations used: CME, chemically modified electrode; CV, cyclic voltammogram; Hb, haemoglobin; PEI, polyethyleneimine; PG, pyrolytic graphite; SCE, saturated calomel electrode.

¹To whom correspondence should be addressed (e-mail genxili@nju.edu.cn).

An unmediated NO biosensor was prepared by co-immobilizing haemoglobin with polyethyleneimine at a pyrolytic graphite electrode surface. Haemoglobin exhibits direct electrochemistry in the membrane, and this modified electrode shows high stability and catalytic activity towards the reduction of NO. A linear relationship between the catalytic current and the concentration of NO was observed in the concentration ranges 1.0×10^-7–8.0×10^-6 M and 1.0×10^-5–2.5×10^-5 M, with a detection limit of 4.0×10^-8 M.

Received 28 March 2003/23 May 2003; accepted 27 May 2003

Published as Immediate Publication 27 May 2003, DOI 10.1042/BA20030056

© 2003 Portland Press Ltd