Medline/PubMed Citation | Related Articles in PubMed | Download to Citation Manager

Biotechnology and Applied Biochemistry (2003) 37, (21–26) (Printed in Great Britain)

PDF

Legacy HTML

A new antibiotic, TH818, and its properties

R. Rachev*¹, V. Gesheva*², T. Tewfike†, S. Bojkova* and R. Zvetkova*

*Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria, and †Botany Department, Agriculture Faculty of Moshtohor, Benha University, El-Kalubia, Egypt

Key words: anti-microbial activity, chromatography, lipopeptide antibiotic, Streptomyces fulvoviolaceus.

Abbreviations used: HPTLC, high-performance thin-layer chromatography; Rt, retention time.

¹Deceased.

²To whom correspondence should be addressed (e-mail veleca@hotmail.com).

Antibiotic TH818 was isolated and purified from a culture broth of Streptomyces fulvoviolaceus 818 by extraction and reversed-phase liquid chromatography. The antibiotic TH818 possesses high molecular mass and unique structure, constructed from carbohydrates, amino acids and lipids. The hydrolysis experiments showed the presence of six fatty acids (hexadecanoic, hexadecenoic, octadecanoic, iso-nonadecanoic, docosanoic and pentacosanoic acids), four sugars (glucose, galactose, N-acetylglucosamine and N-acetylgalactosamine), and seven amino acids (threonine, alanine, glutamic acid, glycine, serine and two unidentified). TH818 has a broad spectrum of anti-microbial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi.

Received 28 June 2002/23 September 2002; accepted 7 October 2002

Portland Press Ltd © 2003