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Volume 37, Part 3
June 2003




Contents
Editorial
Review
Minireview
Plasmid DNA Research Papers
Regular Papers


Editorial
A message from Professor Parviz A. Shamlou
P. A. Shamlou........205

Review
Scaleable processes for the manufacture of therapeutic quantities of plasmid DNA
P. A. Shamlou........207–218
Published as Immediate Publication 28 March 2003, DOI 10.1042/BA20030011

Minireview
Perspectives on plasmid-based gene therapy: challenges for the product and the process
W. J. Kelly........219–223
Published as Immediate Publication 3 April 2003, DOI 10.1042/BA20030033

Plasmid DNA Research Papers
The influence of physico-chemical and process conditions on the physical stability of plasmid DNA complexes using response surface methodology
C. N. Mount, L. K. Lee, A. Yasin, A. Scott, T. Fearn; and P. A. Shamlou........225–234
Published as Immediate Publication 9 April 2003, DOI 10.1042/BA20030012

Optimizing alkaline lysis for DNA plasmid recovery
M. Clemson and W. J. Kelly........235–244
Published as BJ Immediate Publication 3 March 2003, DOI 10.1042/BA20030002

Large-scale capture and partial purification of plasmid DNA using anion-exchange membrane capsules
S. Zhang, A. Krivosheyeva and S. Nochumson........245–249
Published as Immediate Publication 26 March 2003, DOI 10.1042/BA20030009

An apparatus for electrophoretic capture and recovery of circular DNA in thin layers
K. D. Cole........251–257
Published as Immediate Publication 1 April 2003, DOI 10.1042/BA20030003

Evaluation of an ion-exchange membrane for the purification of plasmid DNA
H. N. Endres, J. A. C. Johnson, C. A. Ross, J. K. Welp and M. R. Etzel........259–266
Published as Immediate Publication 11 April 2003, DOI 10.1042/BA20030025

Effect of Pluronic-block copolymers on the reduction of serum-mediated inhibition of gene transfer of polyethyleneimine–DNA complexes
J.-H. S. Kuo........267–271
Published as Immediate Publication 21 February 2003, DOI 10.1042/BA20020123

Regular Papers


Bifunctional constructs of aspirin and ibuprofen (non-steroidal anti-inflammatory drugs; NSAIDs) that express antibacterial and alkylation activities
R. Bartzatt, S. L. G. Cirillo, J. D. Cirillo and L. Donigan........273–282
Published as Immediate Publication 17 January 2003, DOI 10.1042/BA20020108

Purification of capsular polysaccharide from Streptococcus pneumoniae serotype 23F by a procedure suitable for scale-up
V. M. Gonc¸alves, M. Takagi*, R. B. Lima*, H. Massaldi, R. C. Giordano and M. M. Tanizaki........283–287
Published as Immediate Publication 6 January 2003, DOI 10.1042/BA20020075

A rapid and sensitive fluorometric assay method for the determination of nitrilase activity
A. Banerjee, R. Sharma and U. C. Banerjee........289–293
Published as Immediate Publication 28 January 2003, DOI 10.1042/BA20020106

Purification and properties of a transglutaminase produced by a Bacillus circulans strain isolated from the Amazon environment
L. H. de Barros Soares, F. Assmann and M. A. Z. Ayub........295–299
Published as Immediate Publication 15 January 2003, DOI 10.1042/BA20020110

High-yield expression of properly folded insulinoma-associated protein intracellular domain (IA-2ic) in Escherichia coli
M. P. Sica, M. E. Primo, M. R. Ermácora and E. Poskus........301–309
Published as Immediate Publication 6 January 2003, DOI 10.1042/BA20020090

Biosynthesis of rifamycin SV by Amycolatopsis mediterranei MTCC17 in solid cultures
P. S. M. Krishna, G. Venkateswarlu, A. Pandey and L. V. Rao........311–315
Published as Immediate Publication 27 January 2003, DOI 10.1042/BA20020086

Continuous production of a peptidic fraction containing the intermediate opioid peptide LVV-haemorphin-7 (LVVh-7) by peptic hydrolysis of bovine haemoglobin in a continuous membrane reactor
R. Kapel, R. Froidevaux, N. Nedjar-Arroume, A. Fertin-Bazus, P. Dhulster and D. Guillochon........317–324
Published as Immediate Publication 31 January 2003, DOI 10.1042/BA20020078



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