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Biotechnology and Applied Biochemistry (2002) 36, (119–125) (Printed in Great Britain)

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Production of bacteriocins from Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B-5627 using mussel-processing wastes

Nelson Pérez Guerra and Lorenzo Pastrana Castro¹

Departamento de Bioquímica, Xenética e Inmunoloxía, Facultade de Ciencias de Ourense, Universidade de Vigo, As Lagoas, 32004 Ourense, Spain

Key words: lactic acid bacteria, nisin, pediocin, pharmaceutical, veterinary.

Abbreviations used: CDW, cell dry weight; LAB, lactic acid bacteria.

¹To whom correspondence should be addressed (e-mail pastrana@uvigo.es).

The growth and bacteriocin production by Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B-5627 were investigated on mussel-processing wastes. Both bacteriocin productions were satisfactorily modelled using a modified form of the Luedeking and Piret expression, which includes a term for the influence of the pH reduction rate. Experimental data from cultures buffered at different initial concentrations (0, 0.03, 0.10 and 0.25 M) of both bacteria were used to fit and verify the model. The influence of total sugars, nitrogen, phosphorus and buffer concentration on nisin and pediocin production was also studied using response-surface methodology and empirical modelling. Enhanced nisin production (33 BU/ml) was achieved in media buffered with 0.10 M potassium hydrogen phthalate/NaOH. However, the highest levels of pediocin (368 BU/ml) were obtained in the non-buffered media.

Received 12 April 2002/15 May 2002; accepted 11 June 2002

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