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Biotechnology and Applied Biochemistry (2002) 36, (41–45) (Printed in Great Britain)

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Development of an optimized, simple chemically defined medium for bacterial cellulose production by Acetobacter sp. A9 in shaking cultures

Moon-Soo Heo* and Hong-Joo Son¹

*Faculty of Applied Marine Science, Cheju National University, Jeju 690-756, South Korea, and Department of Biotechnology, Miryang National University, Miryang 627-702, Kyungnam, South Korea

Key words: complex medium, ethanol, fermentation, synthetic medium.

Abbreviations used: BC, bacterial cellulose; HS, Hestrin and Schramm.

¹To whom correspondence should be addressed (e-mail shjoo@arang.miryang.ac.kr).

The genus Acetobacter can synthesize cellulose when grown in an undefined medium containing glucose. By using the technique of the omission of a single medium component, an optimized and simple chemically defined medium was developed to support cellulose production by Acetobacter sp. A9 in shaking culture. It contained 4.0% (w/v) glucose, 0.2% (w/v) (NH₄)₂SO₄, 0.25% (w/v) KH₂PO₄, 0.3% (w/v) Na₂HPO₄ ·12H₂O, 0.05% (w/v) MgSO₄ · 7H₂O, 0.0002% (w/v) FeSO₄ · 7H₂O, 0.00025% (w/v) H₃BO₃, 0.00006% (w/v) nicotinamide, 0.00025% (w/v) inositol and 1.4% (v/v) ethanol. A maximum cellulose concentration of around 8 g/l was achieved after 9 days of cultivation at 200 rev./min. The production of cellulose by Acetobacter sp. A9 was greater in simplified synthetic medium than in complex medium (Hestrin and Schramm medium) conventionally used for Acetobacter strains.

Received 12 March 2002/3 May 2002; accepted 13 May 2002

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