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Biotechnology and Applied Biochemistry (2001) 34, (33–36) (Printed in Great Britain)

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A novel method of isolation of a bone-morphogenetic-protein-like protein from ossein

J. Mythili¹, S. Padmavathy and Gowri Chandrakasan

Department of Biochemistry, Central Leather Research Institute, Adyar Chennai 600020, India

Key words: bone-graft device, bovine, osteogenin.

Abbreviations used: BMP, bone morphogenetic protein; BGD, bone-graft device; FTIR, Fourier-transform IR; HPMC, hydroxypropylmethylcellulose.

¹Present address and address for correspondence: Klinik und poliklinik fur Mund und Kiefer, Gesichtschirurgie, AG Biomineralisation, Walderstrasse 30, 48149 Munster, Germany (e-mail mythly_21@yahoo.com).

A new bone-morphogenetic-protein (BMP)-like protein has been isolated through a new protocol from a novel source, ossein. The BMP-like protein was hydrophilic and characterized through Fourier-transform IR studies, SDS/PAGE and coupled with a neutral binder, hydroxypropylmethylcellulose (HPMC) for control release. The IR spectrum of the protein showed peaks in tandem with BMP from bone matrix, and its molecular mass was in the range 18–21 kDa. Sustained release from the surface of HPMC was achieved for a period of 3 days.

Received 10 August 2000/11 December 2000; accepted 20 March 2001

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