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Volume 34, Part 3
December 2001

Contents
Editorial
Regular Papers

Editorial
Approach to assay validation for the development of biopharmaceuticals
R. L. Lundblad........195197
First published on the Internet 22 November 2001
Regular Papers
An associated process for the purification of immunoglobulin G, catalase,
superoxide dismutase and albumin from haemolysed human placenta blood
S. Grellet, E. A. L. Martins, V. M. Gonçalves, A. P. Y. Lopes, I. Raw and J. Cabrera-Crespo........135142
First published on the Internet 9 October 2001
In vitro phagocytosis of carrier mouse red blood cells is
increased by Band 3 cross-linking or diamide treatment
J. A. Jordán, F. J. Alvarez, L. A. Lotero, A. Herráez, J. C. Díez and M. C. Tejedor........143149
First published on the Internet 11 October 2001
Identification of an import signal for, and the nuclear localization of,
human lactoferrin
S. Penco, S. Scarfi, M. Giovine, G. Damonte, E. Millo, B. Villaggio, M. Passalacqua, M. Pozzolini, C. Garrè
and U. Benatti........151159
First published on the Internet 26 October 2001
Efficient cryopreservation of dendritic cells transfected with cDNA of a tumour
antigen for clinical application
G. Pecher, T. Schirrmann, L. Kaiser and J. A. Schenk........161166
First published on the Internet 26 October 2001
Cold lability of lactate dehydrogenase isoenzymes and the
effective preparation of reference material for clinical
laboratory use
K. Yoshikuni, T. Matsuda, W. L. Xia, M. Inagaki, M. Nishimura and K. Tanishima........167171
First published on the Internet 2 November 2001
A simple method for the two-step preparation of two pure
haemorphins from a total haemoglobin peptic hydrolysate by
conventional low-pressure chromatographies
L. Choisnard, D. Durand, D. Vercaigne-Marko, N. Nedjar-Arroume,
P. Dhulster and D. Guillochon........173181
First published on the Internet 2 November 2001
Optimization of the anti-(human CD3) immunotoxin DT389scFv(UCHT1) N-terminal sequence to yield a homogeneous protein
J. M. Hexham, V. King, D. Dudas, P. Graff, M. Mahnke, Y. K. Wang, J.-F. Goetschy, D. Plattner, M. Zurini, F. Bitsch, P. Lake and M. E. Digan........183187
First published on the Internet 22 November 2001
Transphosphatidylation by immobilized phospholipase D in aqueous media
N. Dittrich and R. Ulbrich-Hofmann........189194
First published on the Internet 22 November 2001
Identification of the ligand-binding domain of human vascular-endothelial-growth-factor receptor Flt-1
L. Ma, X. Wang, Z. Zhang, X. Zhou, A. Chen and L. Yao........199204
First published on the Internet 3 December 2001
Studies on the inactivation of soluble and immobilized papain by the ascorbic acidCu2+ system: a model to propose the effect of free radicals on membrane-bound enzymes in vivo
S. Hussain, R. Noor and J. Iqbal........205209
First published on the Internet 3 December 2001
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ISSN Print: 0885-4513
ISSN Electronic: 1470-8744
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