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Volume 34, Part 3
December 2001




Contents
Editorial
Regular Papers



Editorial
Approach to assay validation for the development of biopharmaceuticals
R. L. Lundblad........195–197
First published on the Internet 22 November 2001

Regular Papers
An associated process for the purification of immunoglobulin G, catalase, superoxide dismutase and albumin from haemolysed human placenta blood
S. Grellet, E. A. L. Martins, V. M. Gonçalves, A. P. Y. Lopes, I. Raw and J. Cabrera-Crespo........135–142
First published on the Internet 9 October 2001

In vitro phagocytosis of carrier mouse red blood cells is increased by Band 3 cross-linking or diamide treatment
J. A. Jordán, F. J. Alvarez, L. A. Lotero, A. Herráez, J. C. Díez and M. C. Tejedor........143–149
First published on the Internet 11 October 2001

Identification of an import signal for, and the nuclear localization of, human lactoferrin
S. Penco, S. Scarfi, M. Giovine, G. Damonte, E. Millo, B. Villaggio, M. Passalacqua, M. Pozzolini, C. Garrè and U. Benatti........151–159
First published on the Internet 26 October 2001

Efficient cryopreservation of dendritic cells transfected with cDNA of a tumour antigen for clinical application
G. Pecher, T. Schirrmann, L. Kaiser and J. A. Schenk........161–166
First published on the Internet 26 October 2001

Cold lability of lactate dehydrogenase isoenzymes and the effective preparation of reference material for clinical laboratory use
K. Yoshikuni, T. Matsuda, W. L. Xia, M. Inagaki, M. Nishimura and K. Tanishima........167–171
First published on the Internet 2 November 2001

A simple method for the two-step preparation of two pure haemorphins from a total haemoglobin peptic hydrolysate by conventional low-pressure chromatographies
L. Choisnard, D. Durand, D. Vercaigne-Marko, N. Nedjar-Arroume, P. Dhulster and D. Guillochon........173–181
First published on the Internet 2 November 2001

Optimization of the anti-(human CD3) immunotoxin DT389–scFv(UCHT1) N-terminal sequence to yield a homogeneous protein
J. M. Hexham, V. King, D. Dudas, P. Graff, M. Mahnke, Y. K. Wang, J.-F. Goetschy, D. Plattner, M. Zurini, F. Bitsch, P. Lake and M. E. Digan........183–187
First published on the Internet 22 November 2001

Transphosphatidylation by immobilized phospholipase D in aqueous media
N. Dittrich and R. Ulbrich-Hofmann........189–194
First published on the Internet 22 November 2001

Identification of the ligand-binding domain of human vascular-endothelial-growth-factor receptor Flt-1
L. Ma, X. Wang, Z. Zhang, X. Zhou, A. Chen and L. Yao........199–204
First published on the Internet 3 December 2001

Studies on the inactivation of soluble and immobilized papain by the ascorbic acid–Cu2+ system: a model to propose the effect of free radicals on membrane-bound enzymes in vivo
S. Hussain, R. Noor and J. Iqbal........205–209
First published on the Internet 3 December 2001



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