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Biotechnology and Applied Biochemistry (2001) 33, (127–132) (Printed in Great Britain)

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A rapid method for detecting conformational changes during differentiation and apoptosis of HL60 cells by Fourier-transform infrared spectroscopy

Jiangbing Zhou*, Zhao Wang*¹, Suqing Sun†, Mingjie Liu* and Hongjun Zhang*

*Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, China, and †Department of Chemistry, Tsinghua University, Beijing, 100084, China

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Key words: FT-IR, glycoprotein, lipid, membrane protein.Abbreviations used: FT-IR, Fourier-transform infrared; ATRA, all-trans retinoic acid; VCR, vircristine; NBT, Nitro Blue Tetrazolium; n_sPO₂^-, symmetric PO₂^- stretching vibration; n_asPO₂^-, asymmetric PO₂^- stretching vibration.

¹ To whom correspondence should be addressed (e-mail zwang@tsinghua.edu.cn).

The conformational changes of HL60 cells during differentiation and apoptosis are still not clearly understood. This study uses a new method, Fourier-transform infrared (FT-IR) spectroscopy, to analyse the changes of HL60 cells. We detected several differences among normal HL60 cells, differentiated cells induced by all-trans retinoic acid (ATRA) and PMA, and apoptotic cells induced by vircristine (VCR). The results suggest that the a-helix content of the membrane protein of differentiated HL60 cells induced by ATRA and PMA increased, and that the b-sheet content of membrane proteins of apoptotic cells induced by VCR also increased. Also, C–O(H)-stretching modes of serine, threonine and tyrosine residues of cell proteins were shown to be distinctly different, but no significant differences were detected between cells differentiated along different lineages by ATRA and PMA.

Received 26 September 2000/2 January 2001; accepted 2 January 2001

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