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Biotechnology and Applied Biochemistry (2001) 33, (1–5) (Printed in Great Britain)

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Optimization of fermentation conditions for the production of bacterial cellulose by a newly isolated Acetobacter sp. A9 in shaking cultures

Hong-Joo Son*¹, Moon-Su Heo†, Yong-Gyun Kim* and Sang-Joon Lee‡

*Department of Biotechnology, Miryang National University 1025-1 Naei-dong, Miryang 627-702, Kyungnam, South Korea, †Faculty of Applied Marine Science, Cheju National University, Cheju 690-756, South Korea, and ‡Department of Microbiology, Pusan National University, Pusan 609-735, South Korea

Abbreviations used: BC, bacterial cellulose; Cel^-, cellulose-negative mutant; CSL, corn steep liquor.

¹ To whom correspondence should be addressed (e-mail shjoo@arang.miryang.ac.kr).

The optimum fermentation conditions for the production of cellulose by a newly isolated Acetobacter sp. A9 were determined by shaken cultures. The strain was able to produce cellulose at 25–30 °C with a maximum at 30 °C. Cellulose production occurred at pH 4.5–7.5 with a maximum at pH 6.5. The improved medium composition was 4% (w/v) glucose, 0.1% (w/v) yeast extract, 0.7% (w/v) polypeptone and 0.8% (w/v) Na₂HPO₄·12H₂O. Under these culture conditions, 3.8 g/l cellulose was produced after 7 days of cultivation, although this strain produced only 2.2 g/l in the standard medium. The addition of ethanol to the improved medium enhanced cellulose production: in an improved medium containing 1.4% (v/v) ethanol, cellulose production was 15.2 g/l, which was about four times higher than that without ethanol. Addition of ethanol was found to eliminate the spontaneous mutation of Acetobacter sp. A9.

Received 30 August 2000/4 October 2000; accepted 5 October 2000

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