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Biotechnology and Applied Biochemistry (2000) 32, (155–159) (Printed in Great Britain)

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Preparation and characterization of a new bioinorganic composite: collagen and hydroxyapatite

J. Mythili*¹, T. P. Sastry† and M. Subramanian‡

*Biochemistry Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India, †Bioproducts Laboratory, Central Leather Research Institute, Adyar, Chennai 600 020, India, and ‡Orthopaedic Department, Madras Medical College, Chennai 600 003, India

Abbreviations used: FTIR, Fourier-transform infrared; DSC, differential scanning calorimetry.

¹ To whom correspondence should be addressed, (e-mail mythly@lycos.com).

Studies on biomineralization in bone have opened new arenas in the field of fabrication and engineering of bone-graft devices. A new bioinorganic composite was prepared by introducing processed collagenous matrices into the calcifying solutions at pH 7.8–8.0. The calcifying solutions were prepared by using a saturated dicalcium salt solution. Hydroxyapatite grew on the matrix over a period of 3–5 days in a specially designed crystallizing chamber. Changes in biochemical characters and biophysical parameters have given interesting insights into factors affecting and effecting initiation and progression of calcification on organic matrices.

Received 24 July 2000/17 August 2000; accepted 17 August 2000

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