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Biotechnology and Applied Biochemistry (1998) 27, (245–248) (Printed in Great Britain)

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Immobilization of glucose oxidase on poly-(l-lysine)-modified polycarbonate membrane

Shin-ichiro Suye*, Yuji Kumon† and Akira Ishigaki†

*Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Bunkyo, Fukui 910, Japan

Correspondence: Shin-ichiro Suye.

To whom correspondence should be addressed.

The paper describes the immobilization of glucose oxidase on a polycarbonate membrane modified by a urethane coupling with poly-(l-lysine) and activated with glutaraldehyde. The enzymic properties of the immobilized enzyme were investigated and compared with the those of native glucose oxidase. The thermal stability and pH stability of the immobilized glucose oxidase were greater than those of the native enzyme. The membrane was applied as a glucose sensor.

(Received 11 November 1997/5 January 1998; accepted 28 January 1998)

The Biochemical Society and the Medical Research Society © 1998