| Download to Citation Manager

Biotechnology and Applied Biochemistry (1998) 27, (181–188) (Printed in Great Britain)

PDF

Analysis of hybridoma cell culture processes by SDS/gel capillary electrophoresis and matrix-assisted laser desorption ionization–time-of-flight MS

Vadim Klyushnichenko*, Anja Rodenbrock*, Jörg Thömmes*, Maria-Regina Kula*, Holger Heine† and Manfred Biselli†

*Institut für Enzymtechnologie, Heinrich-Heine Universität Düsseldorf, D-52426 Jülich, Federal Republic of Germany

Abbreviations: CE, capillary electrophoresis; CZE, capillary zone electrophoresis; MALDI–TOF-MS, matrix-assisted laser desorption ionization–time-of-flight MS.

Correspondence: Maria-Regina Kula.

To whom correspondence should be addressed.

SDS/gel capillary electrophoresis (SDS/gel CE) and matrix-assisted laser desorption ionization–time-of-flight MS (MALDI–TOF-MS) were employed to analyse changes in the culture broth during batch and continuous cultivation of hybridoma cells. The stability of IgG was analysed by SDS/gel CE and capillary zone electrophoresis (CZE). The results obtained by the new analytical procedures reflect the changes in the cultivation conditions very well, indicating that these tools can be used to follow animal cell culture processes. A new technique to collect fractions of very small volumes during the CZE separation is described, and the successful off-line coupling of CZE and MALDI–TOF-MS for the analysis of biotechnological processes is demonstrated.

(Received 23 July 1997/15 December 1997; accepted 7 January 1998)

The Biochemical Society and the Medical Research Society © 1998