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Biotechnology and Applied Biochemistry (1998) 27, (31–35) (Printed in Great Britain)

Antigenicity of deoxyadenosine 5-monophosphate cross-linked with polyamine

Aftab Ahmad¹, Shama Ahmad² and Asif Ali

Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh 202 002, India

Abbreviations: SLE, systemic lupus erythematosus; ssDNA, single-stranded DNA; dsDNA, double-stranded DNA; dAMP, deoxyadenosine 5-monophosphate.

¹ Present address: Centre for Biotechnology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi-110062, India.

² Present address: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh-202 002, India.

Correspondence: Asif Ali, Department of Biochemistry, J. N. Medical College, Aligarh Muslim University, Aligarh 202 002, India

Deoxyadenosine 5-monophosphate was cross-linked to spermine using glutaraldehyde as cross-linking reagent. The complex thus obtained was characterized by UV absorption spectroscopy, size-exclusion chromatography and CD. The complex had a molecular mass of around 10200 Da. Antibodies were raised against this complex in rabbits and the titre obtained was found to be > 1:3200. The fine specificity of the raised antibodies was checked by competition ELISA. The complex also bound to naturally occurring anti-DNA autoantibodies, suggesting a possible role of the complex in the pathogenesis of systemic lupus erythematosus.

Received 16 May 1997/5 August 1997; accepted 23 September 1997

© 1998 The International Union of Biochemistry and Molecular Biology