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Biotechnology and Applied Biochemistry (1998) 27, (9–17) (Printed in Great Britain)

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Review

Capillary electrophoresis: a versatile family of analytical technique

Graham Kemp

School of Biomedical Sciences, University of St. Andrews, St. Andrews, Fife, KY16 9AL, Scotland, U.K.

Abbreviations: Abbreviations used: CE, capillary electrophoresis; CGE, capillary gel electrophoresis; CZE, capillary zone electrophoresis; EOF, electroendosmotic flow; MECC, micellar electrokinetic capillary chromatography.

Capillary electrophoresis is a sensitive and versatile technique which has emerged over the past 10 years into the forefront of analytical methodology. Its applicability is enhanced by short separation times, facile and rapid methods of development and a requirement for very small amounts of analyte. This article reviews basic principles behind the technique and the range of options which allow the same instrument to be used for the separation and analysis of a wide range of analytes from large biomolecules, such as proteins and nucleic acids, to metal and organic ions while having the capability to resolve enantiomers and non-charged molecules.

Received 7 October 1997; accepted 8 October 1997

© 1998 The International Union of Biochemistry and Molecular Biology