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Volume 27, Part 3
June 1998




Contents
Regular Papers



Regular Papers
Analysis of hybridoma cell culture processes by SDS/gel capillary electrophoresis and matrix-assisted laser desorption ionization-time-of-flight MS
V. Klyushnichenko, A. Rodenbrock, J. Thömmes, M.-R. Kula, H. Heine and M. Biselli..........181

Expression in Escherichia coli of the IpdA gene, protein sequence analysis and immunological characterization of the P64k protein from Neisseria meningitidis
G. Guillén, A. Alvarez, R. Silva, V. Morera, S. González, A. Musacchio, V. Besada, E. Coizeau, E. Caballero, C. Nazabal, T. Carmenate, L.J. González, R. Estrada, Y. Támbara, G. Padrón and L. Herrera..........189

Novel support for membrane enzyme immobilization: gel beads containing polymerized phospholipid vesicles
T. Gotoh, T. Iwanaga, K.-i. Kikuchi and W.E. Bentley..........197

Immobilization of enzymes on poly(vinyl alcohol)-coated perfluorocarbon supports: comparison of techniques for the immobilization of trypsin and a-amylase on poly(vinyl alcohol)-coated solid and liquid perfluorocarbons
H.A. Chase and Y. Yang..........205

Production and characterization of thermostable phenol oxidases of the ascomycete Thermoascus aurantiacus
A. Machuca, H. Aoyama and N. Durán..........217

Protein and enzyme immobilization on non-porous microspheres of polystyrene
C.-W. Wu, J.-G. Lee and W.-C. Lee..........225

Carboxy-group modification: high-temperature activation of charge-neutralized and charge-reversed b-glucosidases from Aspergillus niger
M.H. Rashid and K.S. Siddiqui..........231

Mechanistic studies of the oxidation of a non-phenolic lignin model compound by the laccase/I-hydroxybenzotriazole redox system
K. Li, R.F. Helm and K.-E.L Eriksson..........239

Immobilization of glucose oxidase on poly-(L-lysine)-modified polycarbonate membrane
S.-i. Suye, Y. Kumon and A. Ishigaki..........245

Molecular mass determination for prostate-specific antigen and a1-antichymotrypsin complexed in vitro
W.D. Bedzyk, B. Larsen, S. Gutteridge and R.A. Ballas..........249

Cell-free bioluminescent screening of translation inhibitors
A.Yu. Gorokhovatsky, L.A. Shaloiko, V.S. Bondar, E.S. Vysotski, E.E. Maximov, H. von Doehren and Y.B. Alakhov..........259

Chitosan--membrane interactions and their probable role in chitosan-mediated transfection
S. Venkatesh and T.J. Smith..........265

Author Index
..........269

Subject Index
..........270



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